Human Nephrin Alexa Fluor® 488-conjugated Antibody

Catalog #: FAB42692G Datasheet / COA / SDS

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FAB42692G-100UG

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Human Nephrin Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Nephrin in direct ELISAs.

Source

Monoclonal Mouse IgG2b Clone # 1037720

Purification

Protein A or G purified

Immunogen

Mouse myeloma cell line NS0-derived human Nephrin
Gln23-Thr1029
Accession # O60500

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Nephrin

Nephrin (or renal glomerulus-specific cell adhesion receptor; also NPHS1) is a 185 - 200 kDa member of the Ig superfamily. It is expressed on glomerular podocyte membranes, associates with same-cell Neph1, and forms homomeric zipper-like complexes with Nephrin on adjacent podocytes. Mature human Nephrin is a type I transmembrane glycoprotein 1219 amino acids (aa) in length. It contains a 1033 aa extracellular region and a 165 aa cytoplasmic tail. One potential, soluble splice form is known where aa’s 1056 - 1095 are deleted, eliminating the transmembrane region. Over aa 23 - 1029, human Nephrin shares 84% and 89% aa identity with mouse and dog Nephrin, respectively.

Entrez Gene IDs

4868 (Human); 54631 (Mouse); 64563 (Rat)

Alternate Names

CNF; Nephrin; nephrosis 1, congenital, Finnish type (nephrin); NPHNCNF; NPHS1; Renal glomerulus-specific cell adhesion receptor