Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1053309
View all NF-H Primary Antibodies »

Product Specifications

Immunogen

Natural Neurofilament H (200 kD) Bovine Protein

Specificity

Detects Human and Bovine NF-H in direct ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human NF‑H Antibody

Detection of NF‑H in Human Brain Cerebellum.

NF‑H was detected in immersion fixed paraffin-embedded sections of human brain cerebellum using Mouse Anti-Human NF‑H Monoclonal Antibody (Catalog # MAB31083) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in Purkinjie neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of NF‑H in HeLa human cervical epithelial carcinoma cell line (positive) and Raji human Burkitt's lymphoma cell line (negative) cells.

NF‑H was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line (positive) and Raji human Burkitt's lymphoma cell line (negative) cells using Mouse Anti-Human NF‑H Monoclonal Antibody (Catalog # MAB31083) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of NF‑H in K562 Human Chronic Myelogenous Leukemia Cell Line (Positive) and Raji Human Burkitt's Lymphoma Cell Line (Negative) Cells.

NF‑H was detected in immersion fixed K562 Human Chronic Myelogenous Leukemia Cell Line (Positive) and Raji Human Burkitt's Lymphoma Cell Line (Negative) Cells using Mouse Anti-Human NF‑H Monoclonal Antibody (Catalog # MAB31083) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human NF‑H Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HeLa (positive), K562 (negative) cells and Raji (negative) cells.

Immunohistochemistry

3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain cerebellum

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NF-H

The human Neurofilament Heavy chain, also known as NF-H and NEFH, is a phosphorylated cytoskeletal intermediate filament protein that is expressed in neurons. Neurofilaments are trimers that always contain the 68 kDa NF-L and variably contain 125 kDa NF-M and 200 kDa NF-H. In the region used for immunization, mouse and rat NF-H are each 93% identical to human NF-H.

Long Name

Neurofilament Protein, Heavy Chain

Alternate Names

NEFH, NFH

Entrez Gene IDs

4744 (Human)

Gene Symbol

NEFH

Additional NF-H Products

Product Documents for Human NF‑H Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human NF‑H Antibody

For research use only

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Protocols

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