Human NgR2/NgRH1 Alexa Fluor® 405-conjugated Antibody

Catalog #: AF2776V Datasheet / COA / SDS

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AF2776V-100UG

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All NgR2/NgRH1 Antibodies

Product Details

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human NgR2/NgRH1 Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human NgR2/NgRH1 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross‑reactivity with recombinant human (rh) NgR and rhNgRH2 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human NgR2/NgRH1
Ala28-Leu401
Accession # Q86UN3

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: NgR2/NgRH1

NgR2, also known as reticulon 4 receptor-like 2 and NgR2, is a 60 kDa glycosylphosphatidylinositol-anchored protein that is expressed primarily in the brain. It contains eight leucine-rich repeats that are flanked by cysteine-rich sequences at both the N- as well as the C-terminus. Membrane-anchored NgR2 can be solubilized through the action of phospholipase or an unidentified MT-MMP to generate a 46 kDa soluble receptor. Mature human NgR2 shares 48% aa sequence identity with human NgRH2. It also shares 96% aa sequence identity with mouse and rat mature NgR2.

Long Name

Nogo-66 Receptor-related Protein 2/Nogo-66 Receptor Homolog 1/Reticulon-4 Receptor-like 2

Entrez Gene IDs

349667 (Human); 269295 (Mouse)

Alternate Names

NgR2; NgRH1; reticulon 4 receptor-like 2; RTN4RL2