Human P-Rex1 Alexa Fluor® 532-conjugated Antibody

Catalog #: FAB8076X Datasheet / COA / SDS

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FAB8076X-100UG

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All P-Rex1 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human P-Rex1 Alexa Fluor® 532-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human P-Rex1 in ELISAs and Western Blots.

Source

Monoclonal Mouse IgG2b Clone # 869704

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human P-Rex1
Lys1313-Arg1434
Accession # Q8TCU6

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 532 (Excitation= 534 nm, Emission= 553 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: P-Rex1

PREX1 (phosphoinositol (3,4,5)-dependent Rac exchanger 1), also called P-Rex1, is a 185 kDa G-protein-activated guanine nucleotide exchange factor. It is mainly cytosolic, but also plasma membrane-associated, regulating cell motility and actin dynamics in neutrophils, migrating neurons, adipocytes and platelets. It is upregulated in cancers such as melanoma, prostate and breast. The 1659 amino acid (aa) human P-Rex1 contains DH, PH and tandem DEP GPCR signaling domains, and a PDZ scaffolding domain between aa 49-703. A 956 aa isoform with a 5 aa substitution for aa 1-703 is reported, and a 1561 aa isoform with a 91 aa substitution for aa 1471-1659 is predicted. The human P-Rex1 immunogen (aa 1313-1434) shares 94% and 95% aa sequence identity with mouse and rat P-Rex1, respectively.

Long Name

Phosphatidylinositol-3,4,5-Trisphosphate-dependent Rac Exchange Factor 1

Entrez Gene IDs

57580 (Human); 277360 (Mouse); 311647 (Rat)

Alternate Names

KIAA1415; phosphatidylinositol 34,5-trisphosphate-dependent Rac exchanger 1 protein; phosphatidylinositol-34,5-trisphosphate-dependent Rac exchange factor 1; PRex1; P-Rex1; ptdIns(34,5)-dependent Rac exchanger 1