P67phox (67 kDa phagocyte oxidase; also NCF2 and NADPH oxidase activator 2) is a 67-70 kDa cytosolic member of the NCF2/NOXA1 family of proteins. It is expressed in multiple cell types, including B cells, endothelial cells, phagocytic cells such as neutrophils and macrophages and, to some degree, fibroblasts. Upon phagocytosis, phagocytes generate superoxide for the purpose of killing ingested microbes. This occurs through the formation and activation of a complex termed NADPH oxidase, which consists of membrane-associated cytochrome b558, plus Rac GTPase and three SH3 domain-containing components, p47phox and a p67phox:p40-phox dimer. Human p67phox is 526 amino acids (aa) in length. It contains two TPR repeats that participate in scaffold formation (aa 11-98), an SH3 domain (aa 240-299), an OPR motif that regulates PB1 interactions (aa 351-429), and a C-terminal SH3 domain (aa 457-516). There is one splice variant that shows a deletion of aa 231-293, and multiple point mutations along the length of the molecule. Over aa 355-526, human p67phox shares 76% aa identity with mouse p67phox.
Human p67phox Alexa Fluor® 405‑conjugated Antibody
R&D Systems | Catalog # AF7830V
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Lys355-Val526 (His389Gln)
Accession # P19878
Specificity
Clonality
Host
Isotype
Applications for Human p67phox Alexa Fluor® 405‑conjugated Antibody
Western Blot
Formulation, Preparation, and Storage
Purification
Formulation
Shipping
Stability & Storage
Background: p67phox
Alternate Names
Gene Symbol
UniProt
Additional p67phox Products
Product Documents for Human p67phox Alexa Fluor® 405‑conjugated Antibody
Product Specific Notices for Human p67phox Alexa Fluor® 405‑conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
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- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars