Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all PDZD11 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PDZD11
Met1-His140
Accession # Q5EBL8

Specificity

Detects human PDZD11 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human PDZD2 and recombinant mouse PDZD2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human PDZD11 Antibody

Detection of Human PDZD11 antibody by Western Blot.

Detection of Human PDZD11 by Western Blot.

Western blot shows lysates of MOLT-4 human acute lymphoblastic leukemia cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human PDZD11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5977) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for PDZD11 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human PDZD11 Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: MOLT‑4 human acute lymphoblastic leukemia cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PDZD11

PDZD11 (PDZ domain-containing protein 11; also AIPP1a and PISP) is a 17 kDa cytosolic protein that bears resemblance to members of the MALS/VELIS family of proteins. It is ubiquitously expressed, and appears to target calcium and copper ATPases to basolateral cell membranes. Human PDZD11 is 140 amino acids (aa) in length. It contains but one PDZ domain (aa 47-129) that apparently interacts with the C-terminus of partner proteins. One alternative start site exists that is 32 aa upstream of the standard site. This generates the AIPP1b isoform. Full-length human PDZD11 shares 97% aa identity with mouse PDZD11.

Long Name

PDZ Domain Containing 11

Alternate Names

AIPP1, PDZD11, PDZK11, PISP

Entrez Gene IDs

51248 (Human); 72621 (Mouse); 302422 (Rat)

Gene Symbol

PDZD11

UniProt

Q5EBL8

Additional PDZD11 Products

Product Documents for Human PDZD11 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human PDZD11 Antibody

For research use only

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