Human Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) Alexa Fluor® 532-conjugated Antibody

Catalog #: FAB1825X Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB1825X-100UG

Save 15% on Select RUO Reagents. See Details.

All ERK1/ERK2 Antibodies

Product Details

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) Alexa Fluor® 532-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human ERK1 and ERK2 dually phosphorylated at T202/Y204 or T185/Y187, respectively.

Source

Monoclonal Mouse IgG1 Clone # 798508

Purification

Protein A or G purified

Immunogen

Phosphopeptide containing the ERK1 T202/Y204 site

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 532 (Excitation= 534 nm, Emission= 553 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: ERK1/ERK2

ERK1 and ERK2 (also known as MAPK3 and MAPK1) are 44- and 42-kDa Ser/Thr kinases, respectively. They are part of the Ras-Raf-ERK signal transduction cascade often found downstream of growth factor receptor activation. ERK1 and ERK2 were initially isolated and cloned as kinases activated in response to insulin and NGF. They are expressed in most, if not all, mammalian tissues. Dual threonine and tyrosine phosphorylation activate both ERKs, at Thr202/Tyr204 for human ERK1 and Thr185/Tyr187 for human ERK2. Within the range used as an immunogen, human, mouse, and rat ERK1 share 100% amino acid sequence identity.

Long Name

Extracellular Signal-regulated Kinase 1/2

Alternate Names

ERK1; ERK-1; ERK1/ERK2; ERT2; Extracellular signal-regulated kinase 1; extracellular signal-related kinase 1; HS44KDAP; HUMKER1A; Insulin-stimulated MAP2 kinase; MAPK 1; Microtubule-associated protein 2 kinase; mitogen-activated protein kinase 3; p44erk1; p44-ERK1; p44mapk; p44-MAPK; PRKM3