Human Phospho-LAT (Y161) Alexa Fluor® 350-conjugated Antibody

Catalog #: FAB6334U Datasheet / COA / SDS

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FAB6334U-100UG

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Human Phospho-LAT (Y161) Alexa Fluor® 350-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Phospho-LAT (Y161) in Western blots.

Source

Monoclonal Mouse IgG1 Clone # 627945

Purification

Protein A or G purified

Immunogen

Phosphopeptide containing the human LAT Y161 site
Accession # O43561

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 350 (Excitation= 346 nm, Emission= 442 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: LAT

LAT (Linker for Activation of T cells) is a type III transmembrane lipid raft protein that serves as a scaffold for signaling molecules. Isoforms of 262 (36 kDa) and 233, called long and short, respectively, differ by inclusion/exclusion of aa 114‑142; a 269 aa isoform lacks this region, but includes alternate N-terminal sequence. Upon T-cell antigen receptor activation, LAT is multiply phosphorylated by ZAP-70/Syk protein tyrosine kinases, creating docking sites for SH2 domain-containing proteins. Phospholipase C-gamma docks at Y161, which is pY132 in the short form of LAT. Mutation of this site results in a Th2 autoimmune lymphoproliferative disorder in mice.

Long Name

Linker for Activation of T cells

Entrez Gene IDs

27040 (Human); 16797 (Mouse); 81511 (Rat)

Alternate Names

36 kDa phospho-tyrosine adapter protein; LAT; LAT1,36 kDa phospho-tyrosine adaptor protein; linker for activation of T cells; linker for activation of T-cells family member 1; p36-38; pp36