The DNA damage response activates cell cycle checkpoints to allow time for DNA repair and ensure the fidelity of each cell cycle. One group of proteins that transduce the DNA damage signal is the Rad family. The hRad17 protein is phosphorylated at S635 in cells exposed to multiple types of genotoxic stress, including ionizing radiation (IR) and ultraviolet light (UV).
Human phospho-Rad17 (S635) Antibody
R&D Systems | Catalog # AF1374
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Discontinued Product
AF1374 has been discontinued.
View all Rad17 products.
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
Phosphopeptide containing human Rad17 S635 site
Specificity
Detects human Rad17 when phosphorylated at S635.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human phospho-Rad17 (S635) Antibody
Detection of Human Phospho-Rad17 (S635) by Western Blot.
Western blot shows lysates of MCF-7 human breast cancer cell line mock treated or exposed to 10 Gy IR or 100 J/m2UV-C for 1 hour. PVDF membrane was probed with 0.5 µg/mL Rabbit Anti-Human Phospho-Rad17 (S635) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1374) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-Rad17 (S635) was detected at approximately 92 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U lambda-phosphatase (lambda-PPase) for 30 minutes time. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human phospho-Rad17 (S635) Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: MCF-7 human breast cancer cell line exposed to Gy IR or UV-C
Sample: MCF-7 human breast cancer cell line exposed to Gy IR or UV-C
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS and Sodium Azide with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Rad17
Alternate Names
CCYC, cell cycle checkpoint protein (RAD17), cell cycle checkpoint protein RAD17, FLJ41520, hRad17, R24L, RAD1 (S. pombe) homolog, RAD1 homolog, RAD17 homolog (S. pombe), Rad17-like protein, RAD17Sp, Rad24, RF-C activator 1 homolog, RF-C/activator 1 homolog
Gene Symbol
RAD17
Additional Rad17 Products
Product Documents for Human phospho-Rad17 (S635) Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human phospho-Rad17 (S635) Antibody
For research use only
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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