Human Pro‑BNP Alexa Fluor® 350-conjugated Antibody

Catalog #: FAB3604U Datasheet / COA / SDS

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FAB3604U-100UG

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Pro‑BNP Alexa Fluor® 350-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Pro‑BNP in direct ELISAs and Western blots. No cross-reactivity with recombinant human BNP (aa 103‑134) is observed.

Source

Monoclonal Rat IgG2a Clone # 370104

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human Proâ€‘BNP
His27-His134
Accession # P16860

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 350 (Excitation= 346 nm, Emission= 442 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: BNP

In cardiac tissue brain natriuretic peptide (BNP) is synthesized as 134 amino acid precursor (prepro-BNP), which is cleaved by proteases to form a 26 aa signal peptide and a 108 aa Pro-BNP (aa 27-134). Proteolytic digestion of Pro-BNP results in formation of 76 aa amino-terminal NT-proBNP (aa 27-102) and biologically active 32 aa BNP hormone molecule (aa 103-134). Both proBNP and NTpro-BNP circulate in human plasma and have been proposed as markers for early diagnosis of left ventricular dysfunction as well as prognostic markers of possible cardiac complications at patients with heart failure.

Long Name

Brain Natriuretic Peptide

Entrez Gene IDs

4879 (Human); 18158 (Mouse); 25105 (Rat)

Alternate Names

BNF; BNP; brain type natriuretic peptide; Gamma-brain natriuretic peptide; natriuretic peptide B; natriuretic peptide precursor B; natriuretic peptides B; natriuretic protein; NPPB