Human PUM2 Alexa Fluor® 350-conjugated Antibody

Catalog #: AF7415U Datasheet / COA / SDS

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AF7415U-100UG

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Human PUM2 Alexa Fluor® 350-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human PUM2 in direct ELISAs and Western blots. In direct ELISAs, less than 4% cross-reactivity with recombinant human PUM1 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human PUM2
Pro103-Gly250
Accession # Q8TB72

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 350 (Excitation= 346 nm, Emission= 442 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: PUM2

PUM2 (Pumilio homolog 2/Pumilio-2) is a 121-125 kDa member of the PUF family of RNA-binding peoteins. It is expressed in a variety of cell types, including neurons, skeletal muscle cells, stem cells and germ cells, and plays a role in asymmetric cell division. PUM2 appears to bind to the 3' untranslated region of target mRNAs. By doing so, it causes a conformational change in the RNA, allowing for the subsequent binding of miRNAs and the silencing of protein translation. In germ cells, PUM2 is also reported to complex with NANOSI and SNAPIN, although its function in this complex is unclear. Human PUM2 is 1066 amino acids (aa) in length. It contains a SNAPIN interaction site (aa 1-260), one PUM-HD region (aa 706-1048) and eight consecutive pumilio repeats (aa 726-1022). There are six utilized Ser/Thr phosphorylation sites, and at least two isoform variants. One is 95-96 kDa in size and shows a deletion of aa 574-652, while another shows a deletion of two amino acids; Val829Ile830. Over aa 103-250, human PUM2 shares 95% aa sequence identity with mouse PUM2.

Long Name

Pumilio 2

Entrez Gene IDs

23369 (Human); 80913 (Mouse); 298874 (Rat)

Alternate Names

FLJ36528; KIAA0235PUMH2PUML2; MGC138251; MGC138253; PUM2; PUMH2; pumilio (Drosphila) homolog 2; pumilio homolog 2 (Drosophila); pumilio homolog 2; Pumilio-2; PUML2