Beta-1,3-N-Acetylglucosaminyltransferase 2/B3GNT2 Antibody

R&D Systems | Catalog # AF3960

R&D Systems

Key Product Details

Species Reactivity

Human, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Beta-1,3-N-Acetylglucosaminyltransferase 2/B3GNT2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human beta -1,3-N-acetylglucosaminyltransferase 2/B3GNT2
Lys29-Cys397
Accession # Q9NY97

Specificity

Detects human and rat beta -1,3-N-acetylglucosaminyltransferase 2/B3GNT2 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Beta-1,3-N-Acetylglucosaminyltransferase 2/B3GNT2 Antibody

Detection of Human and Rat beta -1,3-N-acetylglucosaminyl-transferase 2/B3GNT2 antibody by Western Blot.

Detection of Human and Rat beta -1,3-N-acetylglucosaminyl-transferase 2/B3GNT2 by Western Blot.

Western blot shows lysates of human brain tissue and rat testis tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human beta -1,3-N-acetylglucosaminyltransferase 2/B3GNT2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3960) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for beta -1,3-N-acetylglucosaminyltransferase 2/B3GNT2 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Beta-1,3-N-Acetylglucosaminyltransferase 2/B3GNT2 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Human brain tissue and rat testis tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Beta-1,3-N-Acetylglucosaminyltransferase 2/B3GNT2

B3-GNT (also B3GNT-2) is an 80 kDa (45 kDa without glycosylation) type II transmembrane  Golgi enzyme that belongs to the glycosyltransferase 31 family of enzymes. It catalyzes the transfer of N-acetyl glucosamine from UDP-GlcNAc onto Gal beta 3(GlcNAc beta 6)GalNAc-mucin. It functions in the elongation and branching of O-linked oligosaccharide chains of mucin glycoproteins. Over aa 29-397, human B3GNT2 shares 88% aa sequence identity with rat B3GNT2.

Long Name

UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyl-transferase 1

Alternate Names

B3Galt6, Beta3gnt1

Entrez Gene IDs

10678 (Human); 53625 (Mouse)

Gene Symbol

B3GNT2

UniProt

Q9NY97

Additional Beta-1,3-N-Acetylglucosaminyltransferase 2/B3GNT2 Products

Product Documents for Beta-1,3-N-Acetylglucosaminyltransferase 2/B3GNT2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Beta-1,3-N-Acetylglucosaminyltransferase 2/B3GNT2 Antibody

For research use only

Related Research Areas

Glycobiology-related Enzymes

Customer Reviews for Beta-1,3-N-Acetylglucosaminyltransferase 2/B3GNT2 Antibody

There are currently no reviews for this product. Be the first to review Beta-1,3-N-Acetylglucosaminyltransferase 2/B3GNT2 Antibody and earn rewards!

Have you used Beta-1,3-N-Acetylglucosaminyltransferase 2/B3GNT2 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 for a review with an image

$10/€7/£6/$10CAN/¥1110 for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies