Human/Rat IkB-beta Antibody
Human/Rat IkB-beta Antibody Summary
Accession # Q15653
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human IkB‑ beta by Western Blot. Western blot shows recombinant human Ik beta -a, IkB-beta, and IkB-e (20 ng/lane). PVDF mem-brane was probed with 1 µg/mL Mouse Anti-Human/Rat IkB-beta Mono-clonal Antibody (Catalog # MAB3425) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for Human/Rat IkB-beta was detected at approximately 48-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Detection of Human/Rat IkB‑ beta by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Rat IkB-beta Monoclonal Antibody (Catalog # MAB3425) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific band for Human/Rat IkB-beta were detected at approximately 48 kDa and 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
NF kappa B inhibitor beta (IkB-beta ) inhibits the NF kappa B complex by binding and sequestering it in the cytoplasm. Upon stimulation, IkB-beta is phosphorylated on serine residues marking it for degradation by the ubiquitin pathway. Following IkB-beta degradation, the NF kappa B complex is able to translocate to the nucleus and activate transcription.
Citation for Human/Rat IkB-beta Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
ERK5 protein promotes, whereas MEK1 protein differentially regulates, the Toll-like receptor 2 protein-dependent activation of human endothelial cells and monocytes.
Authors: Wilhelmsen K, Mesa K, Lucero J, Xu F, Hellman J
J Biol Chem, 2012-06-15;287(32):26478-94.
Sample Types: Cell Lysates
Applications: Western Blot
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Reviews for Human/Rat IkB-beta Antibody
There are currently no reviews for this product. Be the first to review Human/Rat IkB-beta Antibody and earn rewards!
Have you used Human/Rat IkB-beta Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image