M-Ras/R-Ras3 Antibody

R&D Systems | Catalog # AF3606

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all M-Ras/R-Ras3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human M-Ras
Met1-Leu208 (Lys181Glu)
Accession # O14807

Specificity

Detects human and rat M-Ras/R-Ras3 in Western blots. Does not detect recombinant human R-Ras or TC21 (R-Ras2).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for M-Ras/R-Ras3 Antibody

Detection of Human and Rat M-Ras/R-Ras3 antibody by Western Blot.

Detection of Human and Rat M‑Ras/R‑Ras3 by Western Blot.

Western blot shows lysates of L6 rat myoblast cell line. PVDF membrane was probed with 2 µg/mL Goat Anti-Human/Rat M-Ras/R-Ras3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3606) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human R-Ras, TC21/R-Ras2, and M-Ras/R-Ras3 (2 ng/lane) were included. A specific band for M-Ras/R-Ras3 was detected at approximately 24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for M-Ras/R-Ras3 Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: L6 rat myoblast cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: M-Ras/R-Ras3

Muscle Ras (M-Ras), also known as R-Ras3, is a small GTP-binding protein of the Ras superfamily of GTPases. M-Ras comprises an R-Ras subfamily with fellow members R-Ras and TC21 (R-Ras2). While highly homologous elsewhere, M-Ras differs at its carboxyl terminus from both R-Ras and TC21, suggesting this GTPase may interact with novel signaling effectors.

Alternate Names

MRas, R-RAS3

Entrez Gene IDs

22808 (Human); 17532 (Mouse); 25482 (Rat)

Gene Symbol

MRAS

UniProt

O14807

Additional M-Ras/R-Ras3 Products

Product Documents for M-Ras/R-Ras3 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for M-Ras/R-Ras3 Antibody

For research use only

Citations for M-Ras/R-Ras3 Antibody

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