Human/Rat PIWIL2 Alexa Fluor® 532-conjugated Antibody

Catalog #: AF6558X Datasheet / COA / SDS

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AF6558X-100UG

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Human/Rat PIWIL2 Alexa Fluor® 532-conjugated Antibody Summary

Species Reactivity

Human, Rat

Specificity

Detects human PIWIL2 in direct ELISAs and human and rat PIWIL2 in Western blots. In direct ELISAs, less than 4% cross-reactivity with recombinant human PIWIL4 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human PIWIL2
Lys216-Asp379
Accession # Q8TC59

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 532 (Excitation= 534 nm, Emission= 553 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: PIWIL2

PIWIL2 (PIWI [P-element-induced wimpy testis]-like 2; also HILI) is a 100-110 kDa cytosolic member of the PIWI subfamily, Argonaute family of proteins. It is expressed in the germ line, particularly in premeiotic and early meiotic germ cells, plus mesenchymal stem cells, and in select cancer stem cells such as CD44⁺CD24^- breast cancer cells. PIWIL2 likely silences mobile genetic elements in germ cells, and appears to depress stem cell proliferation by inhibiting the expression of cell cycle genes. Human PIWIL2 is 973 amino acids (aa) in length. It contains one PAZ domain (aa 384-496) and a C-terminal PIWI domain (aa 668-959) that interact with RNA. There are multiple methylation sites on arginines. Two potential splice forms are reported, one that shows a deletion of aa 887-922, and a second that contains a 12 aa substitution for aa 486-801. Over aa 216-379, human PIWIL2 shares 96% aa identity with rat PIWIL2.

Long Name

Piwi-like Protein 2

Entrez Gene IDs

55124 (Human); 57746 (Mouse); 306011 (Rat)

Alternate Names

Cancer/testis antigen 80; CT80; CT80mili; FLJ10351; HILI; HILIMiwi like; MGC133049; MILI; PIWIL1L; PIWIL2; piwil2-like protein; piwi-like 2 (Drosophila); piwi-like protein 2