Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Western Blot, Neutralization

Cited:

Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # 349102
View all Relaxin-2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Relaxin-2
Val23-Cys185
Accession # P04090

Specificity

Detects both the pro and mature forms of human Relaxin-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human (rh) Relaxin-1 or rhRelaxin-3 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human Relaxin‑2 Antibody

Detection of Human Relaxin-2 antibody by Western Blot.

Detection of Human Relaxin-2 by Western Blot.

Western blot shows lysates of human placenta. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human Relaxin-2 Monoclonal Antibody (Catalog # MAB2804) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for Relaxin-2 at approximately 8 kDa and 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

cAMP production Induced by Relaxin‑2 and Neutralization by Human Relaxin‑2 Antibody.

cAMP production Induced by Relaxin‑2 and Neutralization by Human Relaxin‑2 Antibody.

Recombinant Human Relaxin-2 (Catalog # 3596-RN) induces cAMP production in the THP-1 human acute monocytic leukemia cell line in a dose-dependent manner (orange line), as measured by cAMP Parameter Assay Kit (Catalog # KGE002B). cAMP production elicited by Recombinant Human Relaxin-2 (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Human Relaxin-2 Monoclonal Antibody (Catalog # MAB2804). The ND50 is typically 1.5-6.0 µg/mL.

Applications for Human Relaxin‑2 Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: Human placenta

Neutralization

Measured by its ability to neutralize Relaxin‑2-induced cAMP production in the THP‑1 human acute monocytic leukemia cell line. Parsell, D. A. et al. (1996) J. Biol. Chem. 271:27936. The Neutralization Dose (ND50) is typically 1.5-6.0 µg/mL in the presence of 7.5 ng/mL Recombinant Human Relaxin‑2.

Reviewed Applications

Read 1 review rated 5 using MAB2804 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Relaxin-2

Human Relaxin-2/H2 is a 6 kDa member of the insulin/relaxin gene superfamily. Relaxin-2 is synthesized as a 185 aa preprohormone that is proteolytically processed into a nonglycosylated, disulfide-linked, 53 aa mature heterodimer that contains a 29 aa B chain (aa 25‑53) and a 24 aa A chain (aa 161‑185). An alternate splice form of human Relaxin-2 is known that contains the B chain and an uncleaved 47 aa extension. Mature human Relaxin-2 heterodimer is 48%, 44% and 43% aa identical to rat, canine and porcine Relaxin-2, respectively.

Alternate Names

Relaxin2, RLN2, RLXH2

Entrez Gene IDs

6019 (Human)

Gene Symbol

RLN2

UniProt

P04090

Additional Relaxin-2 Products

Product Documents for Human Relaxin‑2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Relaxin‑2 Antibody

For research use only

Citations for Human Relaxin‑2 Antibody

Customer Reviews for Human Relaxin‑2 Antibody (1)

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  • Human Relaxin-2 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Brain vessels
    Species: Human
    Verified Customer | Posted 09/05/2021
    Human Relaxin‑2 Antibody MAB2804

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