Human ROR2 Antibody

Catalog #: AF2064
6 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2064
AF2064-SP

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Detection of Human ROR2 by Western Blot.

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Product Details

Citations (6)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ROR2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human ROR2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross‑reactivity with recombinant human ROR1 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human ROR2
Val28-Gly403
Accession # AAA60276

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Simple Western

10 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human ROR2 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and T47D human breast cancer cell line. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human ROR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2064) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for ROR2 at approximately 105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western

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Detection of Human ROR2 by Simple Western^TM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for ROR2 at approximately 139 kDa (as indicated) using 10 µg/mL of Goat Anti-Human ROR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2064) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ROR2

ROR2 is a type I receptor tyrosine kinase important in chondrocyte, cartilage and growth plate formation.

Long Name

Receptor Tyrosine Kinase-like Orphan Receptor 2

Entrez Gene IDs

4920 (Human); 26564 (Mouse)

Alternate Names

BDB; BDB1; EC 2.7.10.1; MGC163394; neurotrophic tyrosine kinase receptor-related 2; Neurotrophic tyrosine kinase, receptor-related 2; NTRKR2; NTRKR2tyrosine-protein kinase transmembrane receptor ROR2; receptor tyrosine kinase-like orphan receptor 2; ROR2

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human ROR2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Receptor tyrosine kinase-like orphan receptor 2 (Ror2) expression creates a poised state of Wnt signaling in renal cancer.
Authors: Rasmussen N, Wright T, Brooks S, Hacker K, Debebe Z, Sendor A, Walker M, Major M, Green J, Wahl G, Rathmell W
J Biol Chem, 2013-07-26;288(36):26301-10.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Ror family receptor tyrosine kinases regulate the maintenance of neural progenitor cells in the developing neocortex.
J. Cell. Sci., 2012-02-10;125(0):2017-29.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
Therapeutic Potential and Challenges of Targeting Receptor Tyrosine Kinase ROR1 with Monoclonal Antibodies in B-Cell Malignancies.
Authors: Yang J, Baskar S, Kwong KY
PLoS ONE, 2011-06-15;6(6):e21018.
Species: Human
Sample Types: Recombinant Protein
Applications: ELISA Development
Wnt5a/Ror2-induced upregulation of xPAPC requires xShcA.
Authors: Feike AC, Rachor K, Gentzel M, Schambony A
Biochem. Biophys. Res. Commun., 2010-08-21;400(4):500-6.
Species: Primate - Chlorocebus pygerythrus (Vervet Monkey)
Sample Types: Cell Lysates
Applications: Immunoprecipitation, Western Blot
The Wnt-5a-derived hexapeptide Foxy-5 inhibits breast cancer metastasis in vivo by targeting cell motility.
Authors: Safholm A, Tuomela J, Rosenkvist J, Dejmek J, Harkonen P, Andersson T
Clin. Cancer Res., 2008-10-15;14(20):6556-63.
Species: Human, Mouse
Sample Types: Cell Lysates
Applications: Western Blot
Unique cell surface expression of receptor tyrosine kinase ROR1 in human B-cell chronic lymphocytic leukemia.
Authors: Baskar S, Kwong KY, Hofer T, Levy JM, Kennedy MG, Lee E, Staudt LM, Wilson WH, Wiestner A, Rader C
Clin. Cancer Res., 2008-01-15;14(2):396-404.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry