Human Serpin A12 Alexa Fluor® 700-conjugated Antibody

Catalog #: FAB4410N Datasheet / COA / SDS

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FAB4410N-100UG

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All Serpin A12 Antibodies

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Human Serpin A12 Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Serpin A12 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) Serpin A1, A3, A4, A5, A6, B8, 9, C1, D1, E1, F2, G1, I1, I2, recombinant mouse (rm) Serpin E2, F1, recombinant r

Source

Monoclonal Mouse IgG2a Clone # 484223

Purification

Protein A or G purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Serpin A12
Leu20-Lys414
Accession # Q8IW75

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Serpin A12

Serpin A12 (serine proteinase inhibitor-clade A#12; also known as vaspin) is a 45‑50 kDa secreted member of the alpha 1-antitrypsin (clade A)-subfamily, Serpin superfamily of protease inhibitors. It is produced by visceral adipocytes, and adipocyte-derived stem cells during obesity or diabetes. The presence of vaspin reverses insulin-resistance. Its molecular target is unknown. Human Serpin A12 is 414 amino acids (aa) in length. It contains a 20 aa signal sequence and a 394 aa mature region. A potential "active" inhibitor site lies between Arg363 and Met378. Mature human Serpin A12 shares 61% aa identity with mouse Serpin A12.

Entrez Gene IDs

145264 (Human); 68054 (Mouse); 191570 (Rat)

Alternate Names

antitrypsin), member 12; member 12; OL-64; OL-64Visceral adipose-specific serpin; serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase; Serpin A12; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin); Vaspin; Visceral adipose tissue-derived serine protease inhibitor