Human SIT1 Alexa Fluor® 532-conjugated Antibody

Catalog #: AF5016X Datasheet / COA / SDS

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AF5016X-100UG

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Human SIT1 Alexa Fluor® 532-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human SIT1 in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human SIT1
Gln65-Ser196
Accession # Q9Y3P8

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 532 (Excitation= 534 nm, Emission= 553 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: SIT1

SIT1 (SHP-2-interacting transmembrane adapter 1; also signaling threshold-regulating transmembrane adapter 1) is a 35‑40 kDa type I transmembrane glycoprotein that belongs to a diverse group of transmembrane adapter proteins. It is expressed on thymocytes, plasma cells and T cells. When engaged, SIT1 downregulates TCR signaling. Mature human SIT1 is 172 amino acids (aa) in length. It contains a short extracellular domain (aa 25‑40) that undergoes homodimerization and an extensive intracellular region (aa 62‑196) that contains three key phosphorylation sites (Tyr90/169/188). Over aa 65‑196, human SIT1 is 86% and 80% aa identical to canine and mouse SIT1, respectively.

Long Name

Signaling Threshold Regulating Transmembrane Adaptor 1

Entrez Gene IDs

27240 (Human)

Alternate Names

gp30/40; MGC125908; MGC125909; RP11-331F9.5; SHP2 interacting transmembrane adaptor; SHP2-interacting transmembrane adapter protein; SHP2-interacting transmembrane adaptor protein; signaling threshold regulating transmembrane adaptor 1; signaling threshold-regulating transmembrane adapter 1; SIT1; SITMGC125910; suppression inducing transmembrane adaptor 1; Suppression-inducing transmembrane adapter 1