Human Skp2 Antibody (712120)

R&D Systems | Catalog # MAB6817

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 712120
View all Skp2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Skp2
Leu130-Pro350
Accession # Q13309

Specificity

Detects human Skp2 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Skp2 Antibody (712120)

Detection of Human Skp2 antibody by Western Blot.

Detection of Human Skp2 by Western Blot.

Western blot shows lysates of 293T human embryonic kidney cell line, HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, and U2OS human osteosarcoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Skp2 Monoclonal Antibody (Catalog # MAB6817) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). Specific bands were detected for Skp2 at approximately 45 and 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human Skp2 by Simple WesternTM.

Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cells, loaded at 0.2 mg/mL. A specific band was detected for Skp2 at approximately 49 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human Skp2 Monoclonal Antibody (Catalog # MAB6817). This experiment was conducted under reducing conditions and using the 12‑230 kDa separation system.

Applications for Human Skp2 Antibody (712120)

Application
Recommended Usage

Simple Western

20 µg/mL
Sample: HeLa human cervical epithelial carcinoma cells

Western Blot

2 µg/mL
Sample: 293T human embryonic kidney cell line, HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, and U2OS human osteosarcoma cell line

Reviewed Applications

Read 1 review rated 4 using MAB6817 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Skp2

Skp2 (S-phase kinase-associated protein 2) is a 45-49 kDa cyclin A- and CDK2-associated phosphoprotein within the FBXL (F-box/LRR) family of ubiquitin ligases, designated FBXL1. Skp2 contains an F-box domain (amino acids (aa) 94-140) and 8 leucine-rich repeats (LRR) between aa 180 and 403. Isoforms of 424 and 410 aa, designated a and b, respectively, diverge after aa 354. As a component of the SCF (SKP1-Cul1-F-box) complex, Skp2 phosphorylation is required for degradation of CDK inhibitors such as p27/Kip1 and progression from G1 to S phases of the cell cycle. Deregulation in cancer promotes cell cycle progression and cancer growth. Within the region used as an immunogen, human Skp2 shares 84% aa sequence identity with mouse and rat Skp2.

Long Name

S Phase Kinase-associated Protein 2

Alternate Names

FBL1, FBXL1, p45skp2

Entrez Gene IDs

6502 (Human); 27401 (Mouse); 294790 (Rat)

Gene Symbol

SKP2

UniProt

Q13309 (Human)

Additional Skp2 Products

Product Documents for Human Skp2 Antibody (712120)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Skp2 Antibody (712120)

For research use only

Related Research Areas

Cancer Biomarkers
E3 Ubiquitin Ligases

Customer Reviews for Human Skp2 Antibody (712120) (1)

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  • Human Skp2 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Glioma
    Species: Human
    Verified Customer | Posted 08/07/2020
    Human Skp2 Antibody (712120) MAB6817

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