Human Spi-B Alexa Fluor® 647-conjugated Antibody

Catalog #: FAB7576R Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB7576R-100UG

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Spi-B Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Spi-B in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Spi-B is observed.

Source

Monoclonal Mouse IgG2b Clone # 769653

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human Spi-B
Ala7-Leu115
Accession # Q01892

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Spi-B

Spi-B is a 33-45 kDa member of the ets family of transcription factors. It is found in hematopoietic cells such as B cells and plasmacytoid dendritic cells (DC). In transitional B cells, Spi-B promotes their differentiation into follicular (naïve) B cells. In hematopoietic stem cells, Spi-B stimulates the generation of IFN-producing plasmacytoid DC at the expense of T, B and NK cell development. The 262 amino acid (aa) human Spi-B contains a dual transactivation region (aa 1-62) and an ets DNA-binding domain (aa 169-252). One 177 aa variant diverges after aa 162. Over aa 7-115, human Spi-B shares 72% and 70% aa identity with mouse and rat Spi-B, respectively.

Long Name

Transcription Factor Spi [Spleen FFV Proviral Integration Oncogene] B

Entrez Gene IDs

6689 (Human); 272382 (Mouse); 499146 (Rat)

Alternate Names

Spi-B transcription factor (Spi-1/PU.1 related); SpiB; Spi-B; transcription factor Spi-B