Key Product Details

Species Reactivity

Human

Applications

Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all STEAP1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human STEAP1
Met1-His72
Accession # Q9UHE8

Specificity

Detects human STEAP1 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human STEAP1 Antibody

Detection of Human STEAP1 antibody by Western Blot.

Detection of Human STEAP1 by Western Blot.

Western blot shows lysates of LNCaP human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human STEAP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5587) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for STEAP1 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Detection of Human STEAP1 antibody by Simple WesternTM.

Detection of Human STEAP1 by Simple WesternTM.

Simple Western lane view shows lysates of LNCaP human prostate cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for STEAP1 at approximately 40 kDa (as indicated) using 10 µg/mL of Goat Anti-Human STEAP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5587) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human STEAP1 Antibody

Application
Recommended Usage

Simple Western

10 µg/mL
Sample: LNCaP human prostate cancer cell line

Western Blot

1 µg/mL
Sample: LNCaP human prostate cancer cell line

Reviewed Applications

Read 1 review rated 4 using AF5587 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: STEAP1

STEAP1 (six-transmembrane epithelial antigen of the prostate-1) is a 40 kDa protein (predicted) of the STEAP family of metalloreductases. It is expressed mainly at cell‑cell junctions between prostate secretory epithelium, and up‑regulated in prostate and some bladder, colon and ovarian cancers and Ewing’s sarcomas. Human STEAP1 is 339 amino acids (aa) in length. It contains a ferric oxidoreductase domain (aa 119‑265) that includes transmembrane sequences (# 2, 3, 4, and part of 5, out of 6 sequences). Two splice forms diverge at aa 255, terminating after aa 58 or 259. Over aa 1‑71, human and mouse STEAP1 share 68% aa identity.

Long Name

Six Transmembrane Epithelial Antigen of the Prostate 1

Alternate Names

PRSS24

Entrez Gene IDs

26872 (Human); 70358 (Mouse); 297738 (Rat)

Gene Symbol

STEAP1

UniProt

Q9UHE8 (Human)

Additional STEAP1 Products

Product Documents for Human STEAP1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human STEAP1 Antibody

For research use only

Related Research Areas

Cancer Biomarkers
Redox Enzymes

Customer Reviews for Human STEAP1 Antibody (1)

4 out of 5
1 Customer Rating
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  • Human STEAP1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Cancer cell lysates
    Species: Human
    Verified Customer | Posted 02/25/2016
    The antibody worked on Western Blot. A couple of non-specific bands around, but they faint.

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