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SUMF2 Alexa Fluor® 647-conjugated Antibody

Human Sulfatase Modifying Factor 2/
SUMF2 Alexa Fluor® 647-conjugated Antibody

Catalog #: AF2779R Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2779R-100UG

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Human Sulfatase Modifying Factor 2/
SUMF2 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Sulfatase Modifying Factor 2/SUMF2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 45% cross-reactivity with recombinant mouse SUMF2 is observed and less than 1% cross-reactivity with reco
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Sulfatase Modifying Factor 2/SUMF2
Gln26-Leu301
Accession # Q8NBJ7
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Label
Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration
Sample
Western Blot
Optimal dilution of this antibody should be experimentally determined.
 
Immunoprecipitation
Optimal dilution of this antibody should be experimentally determined.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Sulfatase Modifying Factor 2/SUMF2

Mammalian sulfatases have a common CXPXR motif, in which the Cys residue is post-translationally modified to a Calpha-formylglycine (FGly) residue. This modification is essential for catalytic activity of all sulfatases and is catalyzed by FGly generating enzyme (FGE) encoded by sulfatase modifying factor 1 (SUMF1). FGE deficiency causes multiple sulfatase deficiency (MSD), a rare autosomal recessive disorder. SUMF2 does not have FGly enzymatic activity, but may compete with SUMF1 for the same substrate.

Entrez Gene IDs
25870 (Human)
Alternate Names
C-alpha-formyglycine-generating enzyme 2; C-alpha-formylglycine-generating enzyme 2; DKFZp566I1024; DKFZp686I1024; DKFZp686L17160; DKFZp781L1035; FGE2; MGC99485; paralog of the formylglycine-generating enzyme; pFGE; sulfatase modifying factor 2; sulfatase-modifying factor 2; SUMF2

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SUMF2 Alexa Fluor® 647-conjugated Antibody

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