Human SUMO2/3 MAb (Clone 401504)

R&D Systems | Catalog # A-724

Product is in the process of being discontinued.
R&D Systems
Discontinued Product
A-724 has been discontinued. View all SUMO2/3 products.

Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # 401504
View all SUMO2/3 Primary Antibodies »

Product Specifications

Immunogen

Purified, recombinant human SUMO3
Accession # P55854

Specificity

This antibody effectively immunoprecipitates (IP’s) SUMO2 and SUMO3 when used in conjunction with Protein G Agarose. SUMO1 is not recovered.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Applications for Human SUMO2/3 MAb (Clone 401504)

Application
Recommended Usage

Immunoprecipitation

Recommended IP conditions: use 10-20 µg of A-724 to recover SUMOylated proteins from 0.5 – 1 mg of total protein in 1 ml of cellular lysate. Use of Sentrin Protease inhibitors such as SUMO2/3-Vinyl Sulfone may increase yields of recovered SUMOylated substrates.

Formulation, Preparation, and Storage

Formulation

Supplied as a solution in PBS containing Glycerol and Sodium Azide.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at -20 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Storage below -20 °C is not recommended. Do not use past expiration date.

Background: SUMO2/3

Small Ubiquitin­like Modifiers (SUMOs) are a family of small, related proteins that can be enzymatically attached to a target protein by a post­translational modification process termed SUMOylation. Unlike ubiquitination, which targets proteins for degradation, SUMOylation participates in a number of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. All human SUMO proteins share a conserved ubiquitin-like domain and a C-terminal diglycine cleavage/attachment site. Human SUMO1, SUMO2, SUMO3, and SUMO4 are all translated as propeptides, containing C-terminal prosegments following the diglycine motif that marks the end of the mature forms. Following prosegment cleavage, SUMO1, 2, and 3 may then be enzymatically attached to a lysine on a target protein. It is not clear whether SUMO4 is processed in a similar fashion.

Long Name

Small Ubiquitin-like Modifier 2, & 3

Alternate Names

HSMT3, small ubiquitin-like modifier 2, SMT3A, SMT3B, SMT3H2, SUMO2, SUMO3

UniProt

P55854

Additional SUMO2/3 Products

Product Documents for Human SUMO2/3 MAb (Clone 401504)

Certificate of Analysis

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Product Specific Notices for Human SUMO2/3 MAb (Clone 401504)

For research use only

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Protocols

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FAQs

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