Human SYK (SH2D2 Domain) Alexa Fluor® 750-conjugated Antibody

Catalog #: FAB7166S Datasheet / COA / SDS

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FAB7166S-100UG

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Human SYK (SH2D2 Domain) Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human SYK (SH2D2 Domain) in direct ELISAs. In direct ELISAs, 100% cross-reactivity with the SH2D2 domain of recombinant human (rh) ZAP70 is observed and no cross-reactivity with the SH2 domain of rhCSK is observed.

Source

Monoclonal Mouse IgG1 Clone # 720402

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human SYK (SH2D2 Domain)
Trp168-Cys259
Accession # P43405

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Knockout Validated

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: SYK

SYK (spleen tyrosine kinase) is a 70-80 kDa cytoplasmic non-receptor protein tyrosine kinase of the protein kinase superfamily and the SYK/ZAP-70 subfamily of proteins. The 635 amino acid (aa) human SYK contains two SH2 domains (aa 14-106 and aa 168-259), and one protein kinase domain (aa 371-631). A splicing variant produces a second isoform lacking aa 371-631. Within the second SH2 domain (SH2D2), human SYK shares 94% aa sequence identity with mouse and rat SYK. SYK is widely expressed in hematopoietic cells (notably B lymphocytes), where it couples immunoglobulin receptors to downstream events, such as proliferation, differentiation, and phagocytosis.

Long Name

Spleen Tyrosine Kinase

Entrez Gene IDs

6850 (Human); 20963 (Mouse); 25155 (Rat)

Alternate Names

DKFZp313N1010; EC 2.7.10; EC 2.7.10.2; FLJ25043; spleen tyrosine kinaseFLJ37489; SYK; tyrosine-protein kinase SYK