Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all TAF5L Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human TAF5L
Met1-Tyr189
Accession # O75529

Specificity

Detects human TAF5L in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human TAF1 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human TAF5L Antibody

Detection of Human TAF5L antibody by Western Blot.

Detection of Human TAF5L by Western Blot.

Western blot shows lysates of RPMI 8226 human multiple myeloma cell line, 293T human embryonic kidney cell line, and U2OS human osteosarcoma cell line. Gels were loaded with 20 µg of cytoplasmic (Cyto) and 10 µg of nuclear (Nuc) extracts. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human TAF5L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6785) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TAF5L at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human TAF5L Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: RPMI 8226 human multiple myeloma cell line, 293T human embryonic kidney cell line, and U2OS human osteosarcoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TAF5L

TAF5L (TBP-Associated Factors 5-Like; also RNA polymerase II p300-associated factor 65 kDa subunit 5L, PACF65 beta, PAF65 beta and STAF65 beta ) is a 64-66 kDa member of the TAF5 family of proteins. It is a nuclear protein that forms part of the PCAF, STAGA and TFTC histone acetylase complexes. The acetylases within these complexes acetylate hisone H3 within nucleosomes, allowing for the recognition and binding of transcriptional activators. Human TAF5L is 589 amino acids (aa) in length. It contains an N-terminal homodimerization interface (aa 90-198) plus six consecutive WD repeats (aa 266-547). There is one potential splice form that shows a Val substitution for aa 325-589. Over aa 1-189, human TAF5L shares 94% aa identity with mouse TAF5L.

Long Name

TAF5-like RNA Polymerase II p300/CBP-associated Factor-associated factor 65 kDa Subunit 5L

Alternate Names

PAF65B

Entrez Gene IDs

27097 (Human); 102162 (Mouse); 307927 (Rat)

Gene Symbol

TAF5L

UniProt

O75529 (Human)

Additional TAF5L Products

Product Documents for Human TAF5L Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human TAF5L Antibody

For research use only

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