Human Tenascin X Alexa Fluor® 532-conjugated Antibody

Catalog #: FAB6999X Datasheet / COA / SDS

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FAB6999X-100UG

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All Tenascin XB2 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Tenascin X Alexa Fluor® 532-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Tenascin XB2 in ELISAs and Western blots. Also detects Tenascin X in Western blots. In direct ELISAs, no cross-reactivity with recombinant human Tenascin R is observed.

Source

Monoclonal Mouse IgG2a Clone # 766842

Purification

Protein A or G purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Tenascin XB2
Met1-Tyr673
Accession # AAH33740

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 532 (Excitation= 534 nm, Emission= 553 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Tenascin XB2

Tenascin XB2 (TN-XB2; also TN-XB-Short/S and tenascin-X isoform 2) is a 73-75 kDa member of the tenascin family of extracellular matrix molecules. It is expressed by adrenal gland, and represents the use of an intronic promoter that is tied to an internal start site within the TN-XB gene. A duplication of the TN-XB2 region has created a gene called TN-XA that codes for the same ORF but is not translated. Although TN-XB2 has been reported to bind to tropoelastin, plus collagens I, III and V, other studies using soluble fragments of TN-XB report only tropoelastin interaction. Human TN-XB2 is 673 amino acids (aa) in length. It contains four fibronectin type III repeats (aa 82-442), and one C-terminal fibrinogen-like globular region (aa 449-664). Antibodies to TN-XB2 recognize full-length 465 kDA Tenascin X (TN-XB), 75 kDa and 140-150 kDa C-terminal fragments. Although human TN-XB2 shares 84% aa identity with full-length mouse TN-XB in its C-terminus, there does not appear to be an equivalent alternative start site in the mouse gene.

Entrez Gene IDs

7148 (Human); 81877 (Mouse); 415089 (Rat)

Alternate Names

Tenascin XB2; TNXB2