Human TLE1 Alexa Fluor® 350-conjugated Antibody

Catalog #: AF5947U Datasheet / COA / SDS

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AF5947U-100UG

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Human TLE1 Alexa Fluor® 350-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human TLE1 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) TLE3 and rhTLE4 is observed and less than 5% cross-reactivity with rhTLE2 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human TLE1
His11-Asn200
Accession # Q04724

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 350 (Excitation= 346 nm, Emission= 442 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: TLE1

TLE1 (Transducin-Like Enhancer of Split 1; also ESG1 and GRG1) is a member of the WD repeat Groucho/TLE family of transcriptional repressors. It is widely expressed, and is known to antagonize NFkB and TCF (Wnt)-mediated signaling. TLE1 binds to other family members as a heterooligomer, or to itself as a homooligomer. While it possesses no intrinsic DNA-binding activity, it does modulate the activity of bound transcription factors. Human TLE1 is 770 amino acids (aa) in length. It contains a Gln‑rich region that mediates oligomerization (aa 1-131), a CCN domain that contains an NLS and CDC2 kinase site (aa 200-268), and six WD repeats that mediate protein‑protein interaction (aa 470-767). Phosphorylation on Ser will generate 90-93 kDa and 116 kDa forms in SDS‑Page. Potential splice variants exist. There are alternative start sites at Met57 and Met326, a 10 aa insertion after Gly124, and three distinct eight aa substitutions for aa 193-770, 193-199 and 445-770. Over aa 11-200, human TLE1 shares 98% aa identity with mouse TLE1.

Long Name

Transducin-like Enhancer of Split 1

Entrez Gene IDs

7088 (Human); 21885 (Mouse); 362533 (Rat)

Alternate Names

E(Sp1) homolog; enhancer of split groucho 1; ESG; ESG1Enhancer of split groucho-like protein 1; GRG1; TLE1; transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila); transducin-like enhancer of split 1, homolog of Drosophila E(sp1); transducin-like enhancer protein 1