Human TPT1/TCTP Alexa Fluor® 750-conjugated Antibody

Catalog #: AF4648S Datasheet / COA / SDS

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AF4648S-100UG

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All TPT1/TCTP Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TPT1/TCTP Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human TPT1/TCTP in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse TPT1 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human TPT1/TCTP
Met1-Cys172
Accession # P13693

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: TPT1/TCTP

TPT-1 (translationally controlled tumor protein 1; also TCTP, HRF and fortilin) is a 22-23 kDa member of the TCTP family of calcium-binding proteins. It is widely expressed (but not in kidney), and apparently serves as a transcriptional activator (Oct4), calcium transporter, histamine inducer and antiapoptotic, caspase-3 inhibitor. Human TPT-1 is 172 amino acids (aa) in length. It contains little in the way of structural motifs. There are multiple potential splice variants. There is an alternate start site at Met35 that may be accompanied by a deletion of aa 99-172. In addition, there may be either a 25 aa or 16 aa C-terminal extension after Cys172. Over aa 1-172, human TPT-1 is 96% aa identical to mouse TPT-1.

Long Name

Translationally-controlled Tumor Protein 1

Entrez Gene IDs

7178 (Human); 22070 (Mouse); 116646 (Rat)

Alternate Names

FLJ27337; Fortilin; Histamine-releasing factor; HRF; p02; p23; TCTP; TCTPHRFfortilin; TPT1; translationally-controlled tumor protein; tumor protein, translationally-controlled 1