Human TRIM32 Antibody (717801)

R&D Systems | Catalog # MAB6515

R&D Systems
Discontinued Product
MAB6515 has been discontinued. View all TRIM32 products.

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 717801
View all TRIM32 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human TRIM32
Arg105-Lys204
Accession # Q13049

Specificity

Detects human TRIM32 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human TRIM32 Antibody (717801)

Detection of Human TRIM32 antibody by Western Blot.

Detection of Human TRIM32 by Western Blot.

Western blot shows lysates of A172 human glioblastoma cell line, DU145 human prostate carcinoma cell line, and PC-3 human prostate cancer cell line. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human TRIM32 Monoclonal Antibody (Catalog # MAB6515) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TRIM32 at approximately 72 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human TRIM32 Antibody (717801)

Application
Recommended Usage

Western Blot

0.25 µg/mL
Sample: A172 human glioblastoma cell line, DU145 human prostate carcinoma cell line, and PC‑3 human prostate cancer cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TRIM32

TRIM32 (Tripartite motif-containing protein 32; also 72 kDa TAT-interacting protein, and zinc finger protein HT2A) is a 72-82 kDa member of the TRIM/RBCC family of proteins. It is an E3 ligase that is found in structures termed nuclear and cytoplasmic bodies. Cells known to express TRIM32 are diverse and include fibroblasts, keratinocytes, skeletal muscle cells and neurons. TRIM32 ubiquitinates select proteins such as c-myc, Abi2, actin and dysbindin. Human TRIM32 is 653 amino acids (aa) in length. It contains one E3 ligase RING finger domain (aa 20-65), a B-Box type zinc-finger region (aa 103-133), a coiled-coil region (aa 138-197), five NHL repeats (aa 358-646) and three utilized phosphorylation sites (Ser328/335/339). TRIM32 has the potential to form homomultimers. Over aa 105-204, human TRIM32 exhibits 95% aa identity with mouse TRIM32.

Long Name

Tripartite Motif-containing Protein 32

Alternate Names

BBS11, HT2A, LGMD2H, TATIP

Entrez Gene IDs

22954 (Human); 69807 (Mouse); 313264 (Rat)

Gene Symbol

TRIM32

UniProt

Q13049 (Human)

Additional TRIM32 Products

Product Documents for Human TRIM32 Antibody (717801)

Certificate of Analysis

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Product Specific Notices for Human TRIM32 Antibody (717801)

For research use only

Citations for Human TRIM32 Antibody (717801)

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