Human TRIM5 Alexa Fluor® 488-conjugated Antibody

Catalog #: AF3684G Datasheet / COA / SDS

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AF3684G-100UG

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Human TRIM5 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human TRIM5 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse TRIM5 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human TRIM5
Ala2-Gln248
Accession # Q9C035

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: TRIM5

TRIM5 belongs to the large family of TRIM proteins, which are also named RBCC proteins. These proteins contain a RING domain, one or two B-boxes (CHC3H2 zinc finger motifs), and the coiled-coil domain. Different TRIM proteins contain C-terminals that vary in length and composition. They also have different functions and cellular localization. TRIM5 possesses a B30.2 (also known as RFP-like or PRY-SPRY) C-terminal domain and exists as a homotrimer. TRIM5 is a single protein RING finger E3 ubiquitin ligase and has been shown to have antiretroviral activities. Five splice variants that have identical amino-terminal residues 1‑248, but differ in their C-terminal regions have been reported. Human TRIM5 shares 49% amino acid sequence homology with mouse TRIM5.

Long Name

Tripartite Motif-containing 5

Entrez Gene IDs

85363 (Human)

Alternate Names

EC 6.3.2; EC 6.3.2.-; RING finger protein 88; RNF88; RNF88tripartite motif protein TRIM; TRIM5; TRIM5alpha; tripartite motif containing 5; tripartite motif protein TRIM5; tripartite motif-containing 5; tripartite motif-containing protein 5