Human UGRP1/SCGB3A2 Antibody

R&D Systems | Catalog # AF3545

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all UGRP1/SCGB3A2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human UGRP1/SCGB3A2
Phe22-Val93
Accession # Q96PL1

Specificity

Detects human UGRP1/SCGB3A2 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant mouse UGRP1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human UGRP1/SCGB3A2 Antibody

UGRP1/SCGB3A2 in rat lung tissue.

UGRP1/SCGB3A2 in rat lung tissue.

Native rat lung stained for Scgb3a2 (red), co-stained with RTI-40 (green). Image from a verified customer review.
Detection of UGRP1 by Immunohistochemistry

Detection of UGRP1 by Immunohistochemistry

Images of mouse airways treated with buffers set to either pH 7.40 or 6.80. Data were obtained from multiple regions of interest (ROI) per airway from four different mice. A, D, and G: colocalization (yellow) of V0d2 and SCGB3A2. G shows a significantly higher average ratio at pH 7.4. **P = 0.002. B, E, and H: immunolocalization for V0d2 (green). H shows fluorescence is significantly higher when the luminal pH is alkaline. **P = 0.008. C, F, and I: immunolocalization for SCGB3A2 (red). I shows that SCGB3A2 is pH insensitive. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39254087), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human UGRP1/SCGB3A2 Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human UGRP1/SCGB3A2

Reviewed Applications

Read 2 reviews rated 5 using AF3545 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: UGRP1/SCGB3A2

UGRP-1, also known as secretoglobin family 3A member 2 (SCGB3A2) and High in Normal 2 (HIN-2), is a secreted, 38 kDa, homodimeric protein that is highly expressed in epithelial cells of the airway. UGRP-1 has been shown to bind MARCO (macrophage scavenger receptor with collagenous structure), which is expressed by alveolar macrophages. UGRP1 has been suggested to play a role in lung inflammation. Mature human UGRP1 shares 88% and 81% amino acid sequence identity with rat and mouse mature UGRP1, respectively. Mature UGRP-1 is also 53% identical with that of human mature UGRP-2 (HIN-1).

Long Name

Uteroglobin-related Protein 1

Alternate Names

PnSP-1, SCGB3A2

Entrez Gene IDs

117156 (Human); 117158 (Mouse)

Gene Symbol

SCGB3A2

UniProt

Q96PL1

Additional UGRP1/SCGB3A2 Products

Product Documents for Human UGRP1/SCGB3A2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human UGRP1/SCGB3A2 Antibody

For research use only

Citations for Human UGRP1/SCGB3A2 Antibody

Customer Reviews for Human UGRP1/SCGB3A2 Antibody (2)

5 out of 5
2 Customer Ratings
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 2 的 2 reviews Showing All
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  • Human UGRP1/SCGB3A2 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Lung tissue
    Species: Rat
    Verified Customer | Posted 07/03/2025
    Native rat lung stained for Scgb3a2 (red), co-stained with RTI-40 (green)
    Human UGRP1/SCGB3A2 Antibody AF3545
    Bio-Techne Response
    This review reflects a new species or application tested on a primary antibody.
  • Human UGRP1/SCGB3A2 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: human lung
    Species: Human
    Verified Customer | Posted 01/25/2020
    Human UGRP1/SCGB3A2 Antibody AF3545

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