Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 246904
View all VASA Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human VASA
Met1-Tyr145
Accession # Q9NQI0

Specificity

Detects human VASA in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human VASA Antibody

Detection of Human VASA antibody by Western Blot.

Detection of Human VASA by Western Blot.

Western blot shows lysates of human testis tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human VASA Monoclonal Antibody (Catalog # MAB2030) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for VASA at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human VASA Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Human testis tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VASA

VASA, also known as DDX4, is a member of the DEAD box family of ATP-dependent RNA helicases. The expression of VASA in invertebrate and vertebrate species is restricted to germ cells and is required for germ cell formation. VASA is a highly specific marker of germ cells.

Long Name

DEAD [Asp-Glu-Ala-Asp] Box Polypeptide 41

Alternate Names

DDX4

Entrez Gene IDs

54514 (Human); 13206 (Mouse); 310090 (Rat)

Gene Symbol

DDX4

UniProt

Q9NQI0

Additional VASA Products

Product Documents for Human VASA Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human VASA Antibody

For research use only

Related Research Areas

Primordial and Mature Germ Cells

Citations for Human VASA Antibody

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