Human WIF-1 Alexa Fluor® 350-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: WIF-1

WIF (Wnt Inhibitory Factor) is a secreted protein that binds to Wnt proteins and inhibits their activity. The protein is synthesized as a 379 amino acid (aa) molecule that contains an N-terminal signal sequence, 150 aa WIF domain, 5 EGF-like repeats, and a hydrophilic domain at the carboxy terminus (1). In situ hybridization analysis from the frog, Xenopus laevis, and zebrafish indicate that the message is highly expressed in presomitic mesoderm, the notochord, anterior regions of the brain, branchial arches, nasal placodes, and otic vescicles (1). WIF inhibits secondary axis induction by Wnts and promotes secondary axis induction by Chordin in Xenopus embryos (1). In vitro, WIF binds to Drosophila Wingless and Xenopus Wnt‑8 proteins (1). WIF-1 is implicated as an early event tumor suppressor in cancers of the prostate, breast, lung and bladder (2). However, WIF-1’s role in carcinogenesis may not be that simple since in other cancer types, such as colon adenocarcinoma, WIF facilitates tumorigenesis (3). Human WIF-1 shares 94% and 82% amino acid identity with mouse and frog, respectively.

Product Datasheets

Related Research Areas

• Mesenchymal to Epithelial Transition
• Wnt Inhibitors