Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all ZFP90 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human ZFP90
Lys94-Lys207
Accession # Q8TF47

Specificity

Detects human ZFP90 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant mouse ZFP90 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human ZFP90 Antibody

Detection of Human ZFP90 antibody by Western Blot.

Detection of Human ZFP90 by Western Blot.

Western blot shows lysates of IMR-32 human neuroblastoma cell line and Bowes human melanoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human ZFP90 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7870) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ZFP90 at approximately 72 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human ZFP90 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: IMR‑32 human neuroblastoma cell line and Bowes human melanoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ZFP90

ZFP90 (Zinc Finger Protein 90; also ZFP756 and NK10) is a nuclear 66-72 kDa member of the ZNF505 clade, ZNF91 subfamily, kruppel C2H2-type zinc finger family of proteins. It is widely expressed, and found in cell types such as hepatocytes and cardiac myocytes. Functionally, ZFP90 acts as a transcriptional repressor by interacting with KAP-1/TRIM28/TIF1 beta. This interaction influences histone modifications and heterochromatin protein 1 deposition on DNA. Conversely, it is also known to bind to NRSF, thereby promoting NRSF sensitive gene expression. Human ZFP90 is 636 amino acids (aa) in length. It contains one KRAB domain (aa 14-85) followed by thirteen C2H2-type Zn-finger domains (aa 211-631). Over aa 94-207, human and mouse ZFP90 share 54% aa sequence identity.

Long Name

Zinc Finger Protein 90

Alternate Names

FoxP3-interacting Protein, ZNF756

Entrez Gene IDs

146198 (Human); 22751 (Mouse); 498945 (Rat)

Gene Symbol

ZFP90

UniProt

Q8TF47 (Human)

Additional ZFP90 Products

Product Documents for Human ZFP90 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human ZFP90 Antibody

For research use only

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