Hygromycin B

Tocris Bioscience | Catalog # 4137

Aminoglycoside antibiotic; active against prokaryotic and eukaryotic cells
Tocris Bioscience

Key Product Details

Description

Aminoglycoside antibiotic; active against prokaryotic and eukaryotic cells
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Product Description

Hygromycin B is an aminoglycoside antibiotic. Active against both prokaryotic and eukaryotic cells. Inhibits protein synthesis by binding to the 30S subunit and inhibiting the ribosomal translocation step of elongation.

Product Specifications for Hygromycin B

Molecular Weight

527.52

Formula

C20H37N3O13

Storage

Store at +4°C

Purity

≥90% (HPLC)

Chemical Name

O-6-Amino-6-deoxy-L-glycero-D-galacto-heptopyranosylidene-(1-2-3)-O-β-D-talopyranosyl(1-5)-2-deoxy-N3-methyl-D-streptamine

CAS Number

31282-04-9

PubChem ID

90488914

InChI Key

GRRNUXAQVGOGFE-SZNRJEGWSA-N

SMILES

O[C@H]([C@@H](NC)C[C@@H](N)[C@@H]4O)C4O[C@H]1[C@]2([H])[C@](OC3(O[C@]([H])([C@@H](N)CO)[C@H](O)[C@@H](O)[C@H]3O)O2)([H])[C@@H](O)[C@@H](CO)O1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 50 95

Preparing Stock Solutions for Hygromycin B

The following data is based on the product molecular weight 527.52.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.95 mM 2.00 mL 9.98 mL 19.95 mL
4.75 mM 0.40 mL 2.00 mL 3.99 mL
9.5 mM 0.20 mL 1.00 mL 2.00 mL
47.5 mM 0.04 mL 0.20 mL 0.40 mL

Calculators

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 6 publications citing the usage of this product.

Product Documents for Hygromycin B

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Hygromycin B

For research use only

Customer Reviews for Hygromycin B (1)

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  • Hygromycin used as a selection marker in cells engineered with Hygromycin resistant gene
    Name: Anonymous
    Species: Human
    Assay Type: In Vitro
    Verified Customer | Posted 11/15/2018
    Hygromycin was used as a selection marker for few eukaryotic cells which were engineered with Hygromycin resistant gene. Product works beautifully to inhibit translation and killing non resistant strains giving it desirable specificity.
    Hygromycin B 4137

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