Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
The immunogen this antibody was made to, maps to a region between residue 1582 to 1632 of human KIAA0947 using the numbering given in entry NP_056140.1 (GeneID 23379).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for ICE1 Antibody - BSA Free
Western Blot: ICE1 Antibody [NBP2-32090]
Western Blot: ICE1 Antibody [NBP2-32090] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-KIAA0947 antibody NBP2-32090 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.Immunoprecipitation: ICE1 Antibody [NBP2-32090]
Immunoprecipitation: ICE1 Antibody [NBP2-32090] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-KIAA0947 antibody NBP2-32090 used for IP at 6 ug per reaction. KIAA0947 was also immunoprecipitated by rabbit anti-KIAA0947 antibody BL15136. For blotting immunoprecipitated KIAA0947, NBP2-32090 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.Applications for ICE1 Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
2 - 10 ug/mg lysate
Western Blot
1:1000 - 1:5000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: ICE1
Additional ICE1 Products
Product Documents for ICE1 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for ICE1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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