iCRT 14

Tocris Bioscience | Catalog # 4299

Inhibits β-catenin-responsive transcription (CRT)
Tocris Bioscience

Key Product Details

Description

Inhibits β-catenin-responsive transcription (CRT)
View all beta-Catenin Compounds »

Product Description

iCRT 14 is a potent inhibitor of β-catenin-responsive transcription (CRT) (IC50 = 40.3 nM in assays of Wnt pathway activity). Thought to directly influence the interaction between β-catenin and TCF4. Induces marked G0/G1 cell cycle arrest in HCT-116 and HT29 cell lines. Also acts as transfection enhancer; enhances transgene expression in PC-3 cells by 16 to 35-fold using nonviral systems.

Product Specifications for iCRT 14

Molecular Weight

375.44

Formula

C21H17N3O2S

Storage

Store at +4°C

Purity

≥98% (HPLC)

Chemical Name

5-[[2,5-Dimethyl-1-(3-pyridinyl)-1H-pyrrol-3-yl]methylene]-3-phenyl-2,4-thiazolidinedion

CAS Number

677331-12-3

PubChem ID

5967294

InChI Key

NCSHZXNGQYSKLR-UNOMPAQXSA-N

SMILES

CC(N(C4=CN=CC=C4)C(C)=C3)=C3/C=C(SC2=O)/C(N2C1=CC=CC=C1)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 28.16 75

Preparing Stock Solutions for iCRT 14

The following data is based on the product molecular weight 375.44.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.75 mM 3.55 mL 17.76 mL 35.51 mL
3.75 mM 0.71 mL 3.55 mL 7.10 mL
7.5 mM 0.36 mL 1.78 mL 3.55 mL
37.5 mM 0.07 mL 0.36 mL 0.71 mL

Calculators

Calculate the mass, volume, or concentration required for a solution.

=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Calculate the dilution required to prepare a stock solution.

x
=
x

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background References

References are publications that support the biological activity of the product. See our Citations tab to view 37 publications citing the usage of this product.

Product Documents for iCRT 14

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for iCRT 14

For research use only

Customer Reviews for iCRT 14 (1)

5 out of 5
1 Customer Rating
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used iCRT 14?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 的 1 review Showing All
Filter By:
Clear Filters X
  • Useful Wnt inhibitor
    Name: Anonymous
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: HCT116
    Verified Customer | Posted 07/11/2020
    HCT116 cells were treated with different concentrations of iCRT-14 for 24 hours and the cells were used for transwell assays to investigate the effects of Wnt inhibitor on cell migration.
    iCRT 14 4299

There are no reviews that match your criteria.

Showing  1 - 1 的 1 review Showing All