Key Product Details

Species Reactivity

Mouse

Applications

Immunoprecipitation

Label

PE (Excitation = 488 nm, Emission = 575 nm)

Antibody Source

Monoclonal Rat IgG2A Clone # 355028
View all IL-27 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse IL-27 heterodimer
Tyr19-Pro228 (EBI3) and Phe29-Ser234 (p28)
Accession # O35228 (EBI3) & Q8K3I6 (p28)

Specificity

Detects mouse IL-27 heterodimer in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) IL-27 p28, rmIL‑27 EBI‑3, or recombinant human (rh) IL-27 heterodimer is observed. In Western blots, no cross-reactivity with rmIL-27 p28 or rhIL-27 heterodimer is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for IL-27 Antibody (355028) [PE]

Application
Recommended Usage

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: IL-27

IL27 is encoded by this gene is one of the subunits of a heterodimeric cytokine complex. This protein is related to interleukin 12A (IL12A). It interacts with Epstein-Barr virus induced gene 3 (EBI3), a protein similar to interleukin 12B (IL12B), and forms a complex that has been shown to drive rapid expansion of naive but not memory CD4(+) T cells. The complex is also found to synergize strongly with interleukin 12 to trigger interferon gamma (IFNG) production of naive CD4(+) T cells. The biological effects of this cytokine are mediated by the class I cytokine receptor (WSX1/TCRR).

Long Name

Interleukin 27

Alternate Names

IL27

Gene Symbol

IL27

Additional IL-27 Products

Product Documents for IL-27 Antibody (355028) [PE]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for IL-27 Antibody (355028) [PE]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

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Associated Pathways

Th1 Differentiation Pathway Th1 Differentiation Pathway Thumbnail