Insect Cathepsin L Alexa Fluor® 700-conjugated Antibody

Catalog #: FAB2259N Datasheet / COA / SDS

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FAB2259N-100UG

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Insect Cathepsin L Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity

Insect

Specificity

Detects pro form of Insect Cathepsin L in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with recombinant human (rh) Cathepsin S is observed and no cross-reactivity with rhCathepsin B, F, K, L2/V, and activated,

Source

Monoclonal Mouse IgG2a Clone # 193720

Purification

Protein A or G purified

Immunogen

S. frugiperda ovarian cell line Sf 21-derived Insect Cathepsin L

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Cathepsin L

The pro form of insect cell Cathepsin L is stable at neutral pH, but autoactivates in acidic buffers under reducing conditions. The activated enzyme is similar in substrate specificity to mammalian Cathepsin L, and is very active as a protease, as judged by its ability to degrade azocasein. Cystatins A, B, C, E/M, F, SA, and SN are potent inhibitors of the insect cell Cathepsin L. Like other cysteine proteases related to papain, insect cell Cathepsin L is also inhibited by E-64 and reagents which react with thiols.

Entrez Gene IDs

1514 (Human); 13039 (Mouse); 118276442 (Insect)

Alternate Names

Cathepsin L; cathepsin L1; CATL; CTSL; CTSL1; CTSLEC 3.4.22.15; EC 3.4.22; FLJ31037; Major excreted protein; MEP