IRDye® 680RD, NHS ester

Tocris Bioscience | Catalog # 8878

Near-infared dye (reactive group NHS ester)
Tocris Bioscience

Key Product Details

Description

Near-infared dye (reactive group NHS ester)

Wavelength

Excitation = 680 nm, Emission = 694 nm
View all Fluorescent Dyes »

Product Description

Key information:

IRDye® 680RD, NHS ester is a near-infrared (NIR) fluorescent dye, supplied with an NHS ester reactive group for the labeling of primary amines.

Application: Fluorescence microscopy.

Properties and Photophysical Data: Excitation and emission maxima (λ) are 680 nm and 694 nm, respectively.

Please see the protocol for further information on protein/antibody labeling and conjugation.

NOTE: The choice of solvent for the dye is dependent on the stability of the protein in DMSO. Since a small amount of dye is needed to label large (i.e. >45 kDA) proteins, the amount of DMSO in the reaction is negligible and should not affect protein stability. However, since a proportionally larger amount of dye is required to achieve adequate labeling for a small protein (i.e. <45 kDA), the level of DMSO in the reaction is more likely to affect the protein stability. Dye solubilized in water should be discarded after one use. Dye prepared in DMSO is stable for up to two weeks when stored at -20°C and protected from light and moisture.

It is recommended to dissolve dye in water or DMSO (10 - 20 mg/mL)

IRDye® is a registered trademark of Li-Cor and is used under license.

Optical Data

IRDye<sup>®</sup> 680RD, NHS ester Dye Spectra
λabs 680 nm
λem 694 nm

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Product Specifications for IRDye® 680RD, NHS ester

Molecular Weight

1003.46

Formula

C43H45ClN4Na2O13S3

Storage

Store at -20°C

Purity

≥70% (HPLC)

Chemical Name

Sodium (Z)-2-((2Z,4E)-5-(5-Chloro-3,3-dimethyl-7-(3-sulfonatopropyl)-3H-pyrrolo[2,3-b]pyridin-7-ium-2-yl)-3-(3-(3-((2,5-dioxopyrrolidin-1-yl)oxy)-3-oxopropyl)phenyl)penta-2,4-dien-1-ylidene)-3,3-dimethyl-1-(3-sulfonatopropyl)indoline-5-sulfonate

CAS Number

1373919-87-9

InChI Key

JNFTWCVWRDPJNM-UHFFFAOYSA-L

SMILES

O=S(C1=CC(C/2(C)C)=C(C=C1)N(CCCS(=O)(O[Na])=O)C2=C/C=C(C3=CC(CCC(ON4C(CCC4=O)=O)=O)=CC=C3)/C=C/C5=NC6=[N+](CCCS([O-])(=O)=O)C=C(Cl)C=C6C5(C)C)(O[Na])=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Calculators

Calculate the mass, volume, or concentration required for a solution.

=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Calculate the dilution required to prepare a stock solution.

x
=
x

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background References

References are publications that support the biological activity of the product.

Product Documents for IRDye® 680RD, NHS ester

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for IRDye® 680RD, NHS ester

For research use only

Customer Reviews for IRDye® 680RD, NHS ester

There are currently no reviews for this product. Be the first to review IRDye® 680RD, NHS ester and earn rewards!

Have you used IRDye® 680RD, NHS ester?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card