IRP2 Antibody (9D1-R)

Novus Biologicals | Catalog # NBP3-32483

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 Clone # 9D1-R expressed in HEK293
View all IRP2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein within human IRP2 aa 1-963. (Uniprot: P48200)

Localization

Cytoplasm.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

105 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for IRP2 Antibody (9D1-R)

IRP2 Antibody (9D1-R) Western Blot: IRP2 Antibody (9D1-R) [NBP3-32483]

Western Blot: IRP2 Antibody (9D1-R) [NBP3-32483]

Western blot analysis on different lysates with NBP3-32483 at 1/2,000 dilution. Lane 1: Jurkat cell lysate (20 µg/Lane) Lane 2: A549 cell lysate (20 µg/Lane) Lane 3: Mouse liver tissue lysate (40 µg/Lane) Lane 4: Mouse kidney tissue lysate (40 µg/Lane) Lane 5: Mouse heart tissue lysate (40 µg/Lane) Lane 6: Rat liver tissue lysate (40 µg/Lane) Predicted band size: 105 kDa Observed band size: 105 kDa Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32483 at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Anti-Mouse IgG Secondary Antibody at 1/5,000 dilution was used for 1 hour at room temperature.

Applications for IRP2 Antibody (9D1-R)

Application
Recommended Usage

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH7.4), 0.1% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IRP2

Iron regulatory protein 2 (IRP2) is a central post-transcriptional regulator of cellular and systemic iron metabolism. It is a cytosolic RNA-binding protein that binds to stem-loop structures. IRP2 is regulated by iron-mediated proteolysis. IRP2 also plays a role during hypoxia, as iron and oxygen are closely related.

Alternate Names

ACO3, EC 4.2.1.3, FLJ23381, IRE-BP 2, Iron regulatory protein 2, iron-responsive element binding protein 2, iron-responsive element-binding protein 2, IRP2IRP2AD

Gene Symbol

IREB2

Additional IRP2 Products

Product Documents for IRP2 Antibody (9D1-R)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for IRP2 Antibody (9D1-R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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