Janelia Fluor® 525 ADA
Tocris Bioscience | Catalog # 8962
Key Product Details
Description
Wavelength
Product Description
Key information: Janelia Fluor® 525 ADA is a yellow fluorescent dye suitable for bacterial imaging applications, consisting of a bright Janelia Fluor® fluorophore bearing the unnatural amino acid D-Alanine. Incorporation of fluorescent D-amino acids in gram negative and positive bacteria can selectively label bacterial membranes, resulting in bright fluorescence around the bacteria and minimal or no signal detected inside.Used for: selectively staining Staphylococcus aureus.
Application: confocal microscopy and flow cytometry.
Properties and Photophysical Data: Excitation and emission maxima (λ) are 525 nm and 549 nm, respectively; quantum yield = 0.91; extinction coefficient = 122,000 M-1cm-1; lactone-zwitterion equilibrium constant (KL-Z) = 0.068.
Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research CampusOptical Data
| Emission Color | Yellow |
| λabs | 525 nm |
| λem | 549 nm |
| Extinction Coefficient (ε) | 122,000 M-1cm-1 |
| Quantum Yield (φ) | 0.91 |
| Correction Factor 280 | 0.185 |
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Product Specifications for Janelia Fluor® 525 ADA
Molecular Weight
Formula
Storage
Purity
Chemical Name
InChI Key
SMILES
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 8.41 | 10 |
Preparing Stock Solutions for Janelia Fluor® 525 ADA
The following data is based on the product molecular weight 840.58.
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 11.90 mL | 59.48 mL | 118.97 mL |
| 0.5 mM | 2.38 mL | 11.90 mL | 23.79 mL |
| 1 mM | 1.19 mL | 5.95 mL | 11.90 mL |
| 5 mM | 0.24 mL | 1.19 mL | 2.38 mL |
Calculators
Background References
References are publications that support the biological activity of the product.
There are currently no references for this product.
Product Documents for Janelia Fluor® 525 ADA
Certificate of Analysis
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Product Specific Notices for Janelia Fluor® 525 ADA
For research use only
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