Janelia Fluor® 646, free acid

Tocris Bioscience | Catalog # 6993

Red dye (Reactive group: free acid). Application: flow cytometry, confocal microscopy, super resolution microscopy (SRM) including dSTORM & STED. Suitable for live cell imaging
Tocris Bioscience
Discontinued Product
6993 has been discontinued. An alternative/replacement product is available: 8915. View all Free Acid Janelia Fluor Dyes products.

Key Product Details

Description

Red dye (Reactive group: free acid). Application: flow cytometry, confocal microscopy, super resolution microscopy (SRM) including dSTORM & STED. Suitable for live cell imaging

Wavelength

Excitation = 646 nm, Emission = 664 nm
View all Free Acid Janelia Fluor Dyes »

Product Description

Key Information:

Janelia Fluor® 646, free acid is a red fluorogenic fluorescent dye; supplied with a free acid reactive group. Suitable for live cell imaging.

Application: Suitable for flow cytometry, confocal microscopy, super resolution microscopy (SRM) including dSTORM (in both live and fixed cells) and STED. Used in protocol (2017 Grimm et al - see references below) for the synthesis of Janelia Fluor® HaloTag® and SNAP-Tag® ligands, for use in live cell imaging experiments. Cell permeable.

Properties and Photophysical Data: Excitation and emission maxima (λ) are 646 nm and 664 nm, respectively; quantum yield = 0.54; extinction coefficient = 152,000 M-1cm-1. A280 correction factor = 0.19; lactone-zwitterion equilibrium constant (KL-Z) = 0.0012.

To measure the absorbance spectrum of this dye we recommend the following solvent: ethanol or trifluoroethanol plus 0.1% TFA.

We also offer Janelia Fluor® conjugated antibodies and custom conjugation services with our sister company Novus Biologicals.

HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.

Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.

Licensing Information

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Optical Data

Janelia Fluor<sup>®</sup> 646, free acid Dye Spectra
Emission Color Red
Brightness
λabs 646 nm
λem 664 nm
Extinction Coefficient (ε) 152,000 M-1cm-1
Quantum Yield (φ) 0.54
Closest Laser line 640 nm
Reactive Group Free acid
Reactivity Primary amines
Cell Permeable Yes
Correction Factor 280 0.19
Application Flow Cytometry, Confocal Microscopy, dSTORM, STED, Live-cell Imaging
Alternative For Alexa Fluor® 647, Atto 647, Atto 647N, CF640R, CF647, Cyanine 5

Trademarks and registered trademarks are the property of their respective owners.

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Product Specifications for Janelia Fluor® 646, free acid

Molecular Weight

610.65

Formula

C29H28N2O4Si.CF3CO2H

Storage

Store at -20°C

Purity

≥95% (HPLC)

Chemical Name

1-[7-(1-Azetidinyl)-10-(2,5-dicarboxyphenyl)-9,9-dimethyl-9-silaanthracen-2(9H)-ylidene]azetidinium inner salt trifluoroacetate

InChI Key

NVBSDKPJAFIPEW-UHFFFAOYSA-N

SMILES

OC(C1=CC=C(C([O-])=O)C(C(C2=CC=C(N3CCC3)C=C2[Si]4(C)C)=C(C=C/5)C4=CC5=[N+]6CCC\6)=C1)=O.FC(F)(C(O)=O)F

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMF 12.21 20

Preparing Stock Solutions for Janelia Fluor® 646, free acid

The following data is based on the product molecular weight 610.65.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.2 mM 8.19 mL 40.94 mL 81.88 mL
1 mM 1.64 mL 8.19 mL 16.38 mL
2 mM 0.82 mL 4.09 mL 8.19 mL
10 mM 0.16 mL 0.82 mL 1.64 mL

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Background References

References are publications that support the biological activity of the product.

Product Documents for Janelia Fluor® 646, free acid

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Janelia Fluor® 646, free acid

For research use only

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