JQ1-TAMRA
Tocris Bioscience | Catalog # 8169
Key Product Details
Description
Wavelength
Product Description
Key information: JQ1-TAMRA is an orange fluorescent BET bromodomain ligand for FP and TR-FRET assays. Comprises JQ1 ligand and fluorophore TAMRA, joined by an aliphatic chain. Binds with high affinity to bromodomains BRD3-BD1, BRDT-BD1, BRD2-BD2, BRD3-BD2, BRD4-BD2 and BRD4-BD1 (Kd = 154 nM, 171 nM, 268 nM, 272 nM, 310 nM and 432 nM, respectively).Used for: fluorescence polarization (FP) and TR-FRET assays.
Application: determining binding affinity for BET bromodomains through competition with acylated peptides.
Properties and Photophysical Data: Excitation/emission 554/580 nm; extinction coefficient = 95,000 M-1cm-1; quantum yield = 0.69.
Green probe JQ1-FITC (Cat. No. 7722) also available.
Optical Data
| Emission Color | Orange |
| λabs | 554 nm |
| λem | 580 nm |
| Extinction Coefficient (ε) | 95,000 M-1cm-1 |
| Quantum Yield (φ) | 0.69 |
| Application | TR-FRET, FP |
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Product Specifications for JQ1-TAMRA
Molecular Weight
Formula
Storage
Purity
Chemical Name
InChI Key
SMILES
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 8.55 | 10 |
Preparing Stock Solutions for JQ1-TAMRA
The following data is based on the product molecular weight 855.41.
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 11.69 mL | 58.45 mL | 116.90 mL |
| 0.5 mM | 2.34 mL | 11.69 mL | 23.38 mL |
| 1 mM | 1.17 mL | 5.85 mL | 11.69 mL |
| 5 mM | 0.23 mL | 1.17 mL | 2.34 mL |
Calculators
Background References
References are publications that support the biological activity of the product.
- Chen Viral E protein neutralizes BET protein-mediated post-entry antagonism of SARS-CoV-2. Cell Reports 2022 PMID: 35839775
- Olp Metabolically derived lysine acylations and neighboring modifications tune the binding of the BET bromodomains to histone H4. Biochemistry 2017 PMID: 28945351
Product Documents for JQ1-TAMRA
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for JQ1-TAMRA
For research use only
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