K 114

Key Product Details

Product Description

Key information: K 114 is a potent amyloid fibril-specific fluorescent probe (EC₅₀ = 20 - 30 nM).

Used for: amyloid fibril detection.

Application: fluorescence microscopy, confocal microscopy.

Properties and Photophysical Data: K 114 exhibits minimal fluorescence in aqueous buffers and fluoresces brightly in the presence of Aβ (1-40), α-synuclein and tau in situ. K 114 fluorescence is ph-dependent. Excitation and emission maxima (λ) are 370nm and 450 nm, respectively, at pH 8.5; excitation and emission maxima (λ) are 395nm and 520 nm, respectively, at pH 10.5.

Product Specifications for K 114

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Preparing Stock Solutions for K 114

The following data is based on the product molecular weight 393.27.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

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Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.54 mL	12.71 mL	25.43 mL
5 mM	0.51 mL	2.54 mL	5.09 mL
10 mM	0.25 mL	1.27 mL	2.54 mL
50 mM	0.05 mL	0.25 mL	0.51 mL

Calculators

Background References

References are publications that support the biological activity of the product. See our Citations tab to view 3 publications citing the usage of this product.

• LeVine Mechanism of Aβ(1-40) fibril-induced fluorescence of (trans-trans)-1-bromo-2,5-bis(4-hydroxystyryl)benene (K114). Biochemistry 2005 PMID: 16313197
• Stepanchuk Complex photophysical properties of K114 make for a versatile fluorescent probe for amyloid detection. ACS Chem.Neurosci. 2021 PMID: 33705095
• Crystal A comparison of amyloid fibrillogenesis using the novel fluorescent compound K114. J.Neurochem. 2003 PMID: 12950445

Product Documents for K 114