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Key Product Details
Species
Human
Applications
Western Blot
Product Summary for K-562 Whole Cell Lysate
We recommend adding 1 x sample buffer with 5% BME (or other reducing agent) prior to use.
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Product Specifications
Application Notes
Western Blot Electrophoresis
Specificity
Total Protein - Human Tumor Cell Line: K 562
Type
Cell
Tissue
Blood Lymph
Protein State
Native
Subcellular Fraction
Whole
Formulation, Preparation, and Storage
Formulation
HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors
Concentration
5.0 mg/ml
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store at -80C. Avoid freeze-thaw cycles.
Background: K-562
Additional K-562 Products
Product Documents for K-562 Whole Cell Lysate
Product Specific Notices for K-562 Whole Cell Lysate
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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