L-Ascorbic acid
Tocris Bioscience | Catalog # 4055
Key Product Details
Description
Alternative Names
Product Description
L-Ascorbic acid is an inhibitor of Cav3.2 channels (IC50 = 6.5 μM); displays no effect on Cav3.1 or Cav3.3 channels heterologously expressed in HEK 293 cells. Also enhances the generation of induced pluripotent stem cells (iPSCs) from mouse and human somatic cells by increasing reprogramming efficiency. Commonly used antifade reagent in live cell microscopy. Used for the generation of midbrain dopaminergic neurons from hPSCs or CiPSCs from MEFs (see protocols below). Naturally occurring antioxidant.
L-Ascorbic acid synthesized to Ancillary Material Grade also available.
For more information about how L-Ascorbic acid may be used, see our protocol: Highly Efficient Generation of CiPSCs from MEFs, Generating Midbrain Dopaminergic Neurons from hPSCs.
Product Specifications for L-Ascorbic acid
Molecular Weight
Formula
Storage
Purity
Chemical Name
CAS Number
PubChem ID
InChI Key
SMILES
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 88.06 | 500 |
Preparing Stock Solutions for L-Ascorbic acid
The following data is based on the product molecular weight 176.12.
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 5 mM | 1.14 mL | 5.68 mL | 11.36 mL |
| 25 mM | 0.23 mL | 1.14 mL | 2.27 mL |
| 50 mM | 0.11 mL | 0.57 mL | 1.14 mL |
| 250 mM | 0.02 mL | 0.11 mL | 0.23 mL |
Calculators
Background References
References are publications that support the biological activity of the product. See our Citations tab to view 56 publications citing the usage of this product.
- Cordes Mechanisms and advancement of antifading agents for fluorescence microscopy and single-molecule spectroscopy. Phys.Chem.Chem.Phys. 2011 PMID: 21311807
- Nelson Molecular mechanisms of subtype-specific inhibition of neuronal T-type calcium channels by ascorbate. J.Neurosci. 2007 PMID: 18003836
- Esteban Vitamin C enhances the generation of mouse and human induced pluripotent stem cells. Cell Stem Cell 2010 PMID: 20036631
- Padayatty Vitamin C as an antioxidant: evaluation of its role in disease prevention. J.Am.Coll.Nutr. 2003 PMID: 12569111
Product Documents for L-Ascorbic acid
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for L-Ascorbic acid
For research use only
Related Research Areas
Citations for L-Ascorbic acid
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Customer Images
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Species: HumanAssay Type: In VitroCell Line/Tissue: ipscVerified Customer | Posted 02/08/20200.2 mM
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Species: HumanAssay Type: In VitroCell Line/Tissue: iPSCVerified Customer | Posted 07/17/2018Homemade media for iPSC culture containing L-Ascorbic Acid at a final concentration of 50 ug/ml gives consistent results. Image shows Mapx-5 cell line, containing collagen IV mutations from an Alport Syndrome patient.
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