LGSN Antibody - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-49166
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
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Product Specifications
Immunogen
Produced in rabbits immunized with E. coli-derived Human LGSN fragment
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for LGSN Antibody - Azide and BSA Free
Immunoprecipitation: LGSN Antibody - Azide and BSA Free [NBP3-49166] -
Immunoprecipitation: LGSN Antibody - Azide and BSA Free [NBP3-49166] - Lane A: 0.5 mg HepG2 Whole Cell Lysate. Blotted with 4 uL anti-LGSN rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Immunoprecipitated using NBP3-49166 at 1:100 dilution and HRP-conjugated secondary antibody at 1:1000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 57 kDa. Observed band size: 57 kDaWestern Blot: LGSN Antibody - Azide and BSA Free [NBP3-49166] -
Western Blot: LGSN Antibody - Azide and BSA Free [NBP3-49166] - Lane A: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane using NBP3-49166 at 1:500 dilution. Secondary: HRP-conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 57 kDa. Observed band size: 57 kDa. (We are unsure as to the identity of these extra bands.)Applications for LGSN Antibody - Azide and BSA Free
Application
Recommended Usage
Immunoprecipitation
1-5 uL/mg of lysate
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
PBS (pH 7.0)
Format
Azide and BSA Free
Preservative
0.03% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: LGSN
Alternate Names
GLULD1, glutamate-ammonia ligase (glutamine synthase) domain containing 1, glutamate-ammonia ligase (glutamine synthetase) domain containing 1, Glutamate-ammonia ligase domain-containing protein 1, lengsin, lengsin, lens protein with glutamine synthetase domain, Lens glutamine synthase-like, LGSMGC163240, MGC163238
Gene Symbol
LGSN
Additional LGSN Products
Product Documents for LGSN Antibody - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for LGSN Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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