Lhx4 Antibody (OTI4E11)

Novus Biologicals | Catalog # NBP3-44779

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI4E11
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Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-260 of human LHX4 (NP_203129) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Lhx4 Antibody (OTI4E11)

Lhx4 Antibody (OTI4E11) Western Blot;Lhx4 Antibody (OTI4E11)[NBP3-44779]-

Western Blot;Lhx4 Antibody (OTI4E11)[NBP3-44779]-

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LHX4 (, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LHX4 (1:2000).
Lhx4 Antibody (OTI4E11) Western Blot;Lhx4 Antibody (OTI4E11)[NBP3-44779]-

Western Blot;Lhx4 Antibody (OTI4E11)[NBP3-44779]-

Western blot analysis of extracts (35ug) from HepG2 by using anti-LHX4 monoclonal antibody (1:500).

Applications for Lhx4 Antibody (OTI4E11)

Application
Recommended Usage

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

>95%

Reconstitution

For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized powder ( 1X PBS, pH 7.3,)

Preservative

8% Trehalose

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 degrees C. Avoid freeze/thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Lhx4

Lhx4 encodes a member of a large protein family which contains the LIM domain, a unique cysteine-rich zinc-binding domain. The encoded protein may function as a transcriptional regulator and be involved in control of differentiation and development of the pituitary gland. Mutations in this gene are associated with syndromic short stature and pituitary and hindbrain defects. An alternative splice variant has been described but its biological nature has not been determined.

Alternate Names

CPHD4, FLJ22769, Gsh4, LIM homeobox 4, LIM homeobox protein 4, LIM/homeobox protein Lhx4

Gene Symbol

LHX4

Additional Lhx4 Products

Product Documents for Lhx4 Antibody (OTI4E11)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Lhx4 Antibody (OTI4E11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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